Il17ra Knockout cell line(Pan-02)

Il17ra Knockout cell line(Pan-02) Order

Catalog#

YKO-MJ016

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Il17ra Knockout cell line (Pan-02)

Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Mouse

Gene Name

Il17ra

Gene ID

16172

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10% FBS

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:Pan-02				STR Info (Cell bank) Cell Line:Panc02
Loci	Allele1	Allele2	Allele3	Allele4	Allele1	Allele2	Allele3
45018	237.54 【20.3】				237.64 【20.3】
45051	343.48 【16】	351.68 【18】			343.79 【16】	351.89 【18】
45081	299.78 【18】				299.85 【18】
45084	354.29 【17】				354.44 【17】
45171	229.17 【17】	233.22 【18】			229.29 【17】	233.23 【18】
45261	229.6 【17】				229.7 【17】
45000	200.63 【22.3】				200.81 【22.3】
45003	147.69 【15】				147.66 【15】
X-1	416.33 【29】				417.19 【29】
D4S2408

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Il17ra gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Pan-02 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Il17ra gene knockout Il17ra Knockout cell line (Pan-02) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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